Cov txheej txheem tshuaj tua kab mob thiab ua kom tsis muaj menyuam thaum lub sijhawm hloov HEPA / ULPA cov ntxaij lim dej hauv cov khoom siv biosafety yog ib kauj ruam tseem ceeb ntawm biosafety, tsom rau kev tiv thaiv cov neeg ua haujlwm thiab ib puag ncig thaum rhuav tshem cov lim qub uas yuav kis tau cov kab mob. Phau ntawv qhia no muab cov txheej txheem kev ua haujlwm nruj thiab cov ntsiab lus qhia.
lub hauv paus ntsiab lus
Xav txog tag nrho cov txheej txheem hloov pauv raws li kev ua haujlwm kho rau 'cov cuab yeej muaj peev xwm kis tau'. Tag nrho cov kev ntsuas kev ua haujlwm nyob ib puag ncig "kev ntim khoom, kev ua kom huv, thiab kev tiv thaiv" kom ntseeg tau tias cov pa phem raug tua tau zoo thiab raug kaw thaum lub sijhawm ua haujlwm.
Ntu 1: Kev npaj tshuaj tua kab mob thiab ua kom tsis muaj menyuam ua ntej hloov
Lub hom phiaj ntawm theem no yog txhawm rau txo cov khoom siv roj ntsha hauv lub lim thiab chav, tsim kom muaj kev nyab xeeb disassembly.
1. Kev soj ntsuam kev pheej hmoo thiab kev npaj tsim
Qhia meej txog qib kev pheej hmoo: Txheeb xyuas cov kab mob siab tshaj plaws (xws li BSL-2 lossis BSL-3 cov kab mob) uas lub txee ruaj ntseg tau ua. Txoj kev npaj tshuaj tua kab mob yuav tsum ua raws li qib kev pheej hmoo no.
Xaiv cov tshuaj tua kab mob: Xaiv qhov dav -spectrum, muaj txiaj ntsig, thiab siv tau cov tshuaj tua kab mob raws li hom kab mob. Cov kev xaiv uas muaj xws li:
Cov tshuaj chlorine uas muaj tshuaj tua kab mob (xws li npaj tshiab 1000-5000mg / L cov tshuaj chlorine zoo): muaj zog tua cov kab mob, cov kab mob, thiab cov kab mob fungal, tab sis muaj zog heev. Nws yog ib qho tseem ceeb kom tshem tawm cov residue kom huv si tom qab lub sijhawm sib cuag.
Hydrogen peroxide (xws li 3-6% hydrogen peroxide tov lossis hom sib xyaw): broad-spectrum, low residue, haum rau ntau qhov chaw.
70% -75% ethanol lossis isopropanol: zoo tiv thaiv cov kab mob thiab cov kab mob feem ntau, tab sis txwv tsis pub cov kab mob thiab cov kab mob, feem ntau siv rau kev tua kab mob ua ntej thiab pab tua kab mob.
Phenolic compounds: Dilute thiab siv raws li cov lus qhia.
Txiav txim siab txoj kev tshuaj tua kab mob: feem ntau ua ke "kua so / infusion" thiab "gas / steam fumigation" kom ua tiav cov txiaj ntsig zoo tshaj plaws.
2. Kev ua haujlwm thiab thawj zaug tua kab mob ntawm lub txee ruaj ntseg
Kev ua kom huv huv: Ua ntej kev hloov pauv, cia lub txee ruaj ntseg khiav ntawm qhov load tag nrho tsawg kawg 30 feeb yam tsis muaj kev cuam tshuam (rau siab - cov xwm txheej txaus ntshai, nws raug nquahu kom khiav ntau dua 1 teev), siv cov cua nkag mus ntes cov khoom raug tshem tawm mus rau lub lim.
Deg so thiab disinfection:
Hnav cov cuab yeej tiv thaiv tus kheej (lab tsho, hnab looj tes, tsom iav).
Siv cov tshuaj tua kab mob tsim nyog (xws li 70% cawv) kom huv si so txhua qhov chaw hauv lub txee, suav nrog cov phab ntsa sab hauv, cov rooj zaum ua haujlwm, iav qhov rais, grilles, thiab lwm yam, kom tshem tawm cov kab mob.
Cov kauj ruam tseem ceeb: Kev tua kab mob sib sib zog nqus ntawm qhov chaw ua haujlwm (fumigation method - pom zoo heev)
Qhov no yog qhov ua tau zoo tshaj plaws ua ntej - txoj kev kho mob uas tuaj yeem tua cov kab mob uas tsis tuaj yeem ntes tau los ntawm huab cua thiab muab tso rau hauv cov ces kaum thiab qhov chaw.
Txoj kev: Raws li qhov kev pom zoo ntawm kev ua kom muaj kev nyab xeeb, lub tshuab hluav taws xob "VHP / H2O2" tuaj yeem siv rau kev ncig hauv lub txee, lossis formaldehyde fumigation tuaj yeem siv (vim kev nyab xeeb thiab ib puag ncig teeb meem, tam sim no tsis tshua siv thiab yuav tsum tau tswj nruj heev).
Lwm txoj kev daws teeb meem yooj yim: Yog tias tsis muaj cov cuab yeej tshaj lij, cov ntaub tsis yog - woven npuag soaked nyob rau hauv cov tshuaj tua kab mob siab (xws li hydrogen peroxide) tuaj yeem sib npaug ntawm qhov chaw ua haujlwm tom qab kaw, thiab lub qhov rais pem hauv ntej yuav tsum raug kaw kom muaj kev sib cuag nrog tsawg kawg yog 1 teev, tsim kom muaj qhov chaw ua haujlwm siab hauv zos.
3. Kev npaj siab -cov khoom siv tiv thaiv qib (PPE) rau cov neeg ua haujlwm
Tus neeg tsav tsheb hloov pauv yuav tsum hnav:
Conjoined tiv thaiv khaub ncaws (zoo dua pov tseg thiab ua kua resistant).
Ob chav txheej hnab looj tes (cov hnab looj tes kho mob sab hauv, cov hnab looj tes sab nrauv tuab).
N95 lossis qib siab tiv thaiv daim npog ntsej muag (thaum tuav siab - cov kab mob txaus ntshai, yuav tsum siv lub tshuab lim cua lim cua (PAPR)).
Goggles los yog daim npog ntsej muag tag nrho.
khau npog
Npaj cov hnab ntim khoom pov tseg biohazard, ntim cov tshuaj tua kab mob, thiab cov ntaub nplaum sib khi.
Ntu 2: Kev tua kab mob tiag tiag thiab kev tswj kom muaj kuab paug thaum lub sijhawm hloov txheej txheem
Lub hom phiaj ntawm theem no yog kom ua tiav "zero leakage" lossis "txo qis" thaum dismantling thiab tshem tawm cov lim dej qub.
4. Kev tua kab mob thib ob tom qab kaw thiab lub hwj chim -tawm
Fais fab tuag xauv: Tom qab ua tiav fumigation los yog hu rau tshuaj tua kab mob, disconnect lub hwj chim ntawm lub txee ruaj ntseg thiab xauv nws nrog ib tug tag.
Cov tshuaj tua kab mob thib ob: Tom qab tus neeg teb xov tooj hnav cov PPE tag nrho, qhib lub qhov rais pem hauv ntej thiab so thaj chaw kom rov kov cov tshuaj tua kab mob (xws li lim thav duab, kho cov cuab yeej, cuab yeej tuav).
5. Disassembly thiab qhwv ("lub hnab hauv hnab" thev naus laus zis)
Qhov no yog cov cuab yeej tseem ceeb rau kev tiv thaiv cov pa phem los ntawm kev khiav tawm.
Npaj ob npaug - txheej ntim: Qhib ib lub hnab loj, tuab biohazard pov tseg hauv thaj chaw ua haujlwm txee ruaj ntseg lossis nyob ib sab ntawm nws qhov qhib.
Disassemble lub fixing device: Ua tib zoo tshem tawm lub siab sawb, fixture, thiab lwm yam ntawm lub lim, thiab tsau tag nrho cov me me nyob rau hauv ib lub thawv me me uas muaj tshuaj tua kab mob.
Tshem tawm thiab kaw tam sim:
Ob tug neeg sib koom tes, ib qho ua tib zoo loosening lub lim los ntawm ib sab thiab lwm tus stabilizing nws.
Xaub lub qub lim ncaj qha thiab du rau hauv cov txheej txheem npaj thawj zaug ntawm lub hnab biohazard.
Lub luag haujlwm tseem ceeb: Thaum lub lim tau nkag mus rau hauv lub hnab tag nrho tab sis tseem tsis tau kov hauv qab, pab cov neeg ua haujlwm tam sim ntawd qhwv thiab ceev lub hnab qhib upwards los ntawm sab nraud, txo cov huab cua cuam tshuam hauv lub hnab.
Txau lub hnab qhib nrog tshuaj tua kab mob, thiab tom qab ntawd tus neeg teb xov tooj tseem ceeb txuas lub hnab qhib los ntawm sab hauv nrog zip ties lossis daim kab xev.
Txheej txheej txheej txheej: Muab lub hnab ntim thawj txheej ntim rau hauv lub hnab biohazard thib ob, tshuaj tua kab mob rau ntawm lub hnab qhib, thiab khi kom nruj los ntawm cov neeg ua haujlwm sab nraud.
Deg tshuaj tua kab mob: Tam sim ntawd tsuag tshuaj tua kab mob rau sab nrauv ntawm ob lub hnab - txheej.
6. Kev tua kab mob kawg ntawm kab noj hniav
Tom qab tshem tawm thiab kaw cov lim qub, tam sim ntawd so thiab yaug tag nrho cov chaw sab hauv xws li lub lim teeb tsa, lub thawv siab zoo li qub, kiv cua chamber, thiab lwm yam kom huv si thiab siv tshuaj tua kab mob.
Soak tag nrho cov disassembled inner lining panels, diffuser panels, thiab lwm yam khoom siv tshuaj tua kab mob.
Khaws cov tshuaj tua kab mob nrog lub chamber kom txaus sijhawm (feem ntau 20-30 feeb).
So nrog cov dej tsis muaj dej los yog cov ntaub ntawv tsis muaj lint dawb dipped hauv dej huv kom tshem tawm cov tshuaj tua kab mob (tshwj xeeb tshaj yog cov tshuaj tua kab mob corrosive), thiab qhuav nrog ib daim ntaub huv lossis cua qhuav.
Ntu Peb: Kev Ua Haujlwm thiab Kev Tshawb Fawb Tom Qab Hloov
7. Kev kho zaum kawg ntawm cov lim dej qub
Siv ob npaug - txheej kaw cov lim qub ua cov pov tseg uas muaj kev phom sij heev thiab xa mus rau siab - kom tsis muaj menyuam kom sai li sai tau raws li kev soj ntsuam biosafety.
Lub hnab kom tsis muaj menyuam yuav tsum tau sau meej meej nrog 'Biosafety Txee Khib Nyiab HEPA Filter - Contaminated'.
Nws yog nruj me ntsis txwv tsis pub pov tseg los yog qhib nws li qub pov tseg yam tsis muaj sterilization.
8. Kev teeb tsa thiab kev txheeb xyuas cov lim dej tshiab
Tom qab paub meej tias lub installation chamber tau raug ntxuav thiab qhuav, lub lim tshiab tuaj yeem ntsia tau.
Tom qab kev teeb tsa, tus kws tshaj lij yuav tsum ua "post installation kev ncaj ncees scan xau kuaj", uas yog tib qho kev ntsuas rau kev txheeb xyuas cov txheej txheem kev txhim kho thiab tshuaj tua kab mob kom tsis txhob kis cov lim tshiab. Tsuas yog tom qab dhau qhov kev tshuaj xyuas tuaj yeem rov ua dua lub txee kev nyab xeeb.
Tseem ceeb ceev faj thiab taboos
Kev txwv tsis pub ua haujlwm qhuav: Nws yog nruj me ntsis txwv tsis pub ncaj qha disassemble thiab tshem tawm cov lim yam tsis muaj tshuaj tua kab mob.
Kev txwv tsis pub ib tus neeg ua haujlwm: Ob tus neeg yuav tsum sib koom tes, nrog ib tus neeg ua haujlwm tseem ceeb thiab ib tus neeg pab cuam dhau cov cuab yeej thiab kev sib khi.
Kev txwv tsis pub muaj kev tiv thaiv tsis txaus: Tsis muaj qhov xwm txheej yuav tsum tau txo qis PPE cov qauv.
Kev tswj ib puag ncig: Ua nyob rau hauv qhov chaw ua pa zoo, zoo dua nyob hauv thaj chaw tseem ceeb ntawm chav kuaj biosafety.
Txoj kev npaj khomob kub ntxhov: Npaj cov khoom txeej txeej thiab qhia meej txog cov txheej txheem thaum muaj xwm txheej ceev thaum muaj kev sib kis tsis zoo (xws li lub hnab tawg).
Cov txheej txheem xaus
Txheeb xyuas cov kev pheej hmoo → Xaiv cov tshuaj tua kab mob → Fumigation / tshuaj tua kab mob hauv lub txee → Cov neeg ua haujlwm siab tshaj kev tiv thaiv → Fais fab tuag → Cov tshuaj tua kab mob thib ob → Tshem tawm cov lim dej qub hauv ib lub hnab → Cov tshuaj tua kab mob kawg ntawm lub chamber → Kev kub siab sterilization ntawm lub qub lim → Txhim kho cov lim tshiab → Yuav tsum tau kuaj xyuas thiab kuaj xyuas.
Rau feem coob ntawm cov chaw soj nstuam, tshwj xeeb tshaj yog cov uas cuam tshuam nrog BSL-2 thiab cov kab mob saum toj no, nws tau pom zoo kom outsource qhov haujlwm no rau biosafety txee cov chaw muab kev pabcuam nrog kev nplua nuj thiab cov khoom siv tshuaj tua kab mob. Lawv tuaj yeem muab cov txheej txheem kev nyab xeeb kev ua haujlwm, cov cuab yeej fumigation kev tshaj lij, thiab dais cov kev pheej hmoo biosafety, ua rau lawv muaj kev ntseeg siab dua thiab ua raws li kev xaiv.
